Molecular characterization of pestiviruses isolated from bovines in Chile.

نویسندگان

  • José Pizarro-Lucero
  • María-Orfelia Celedón
  • Marcela Aguilera
  • Alejandra de Calisto
چکیده

Thirty-three pestiviruses isolated from bovines on different farms in Chile were characterized at the molecular level. The 5'-untranslated region (5'UTR) of the isolates was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and subsequently digested with restriction enzymes (RE) Bgl I, Xho I and Pst I. Furthermore, the isolates were amplified by differential RT-PCR, which selectively amplified bovine viral diarrhea virus type 2 (BVDV-2) but not bovine viral diarrhea virus type 1 (BVDV-1). Of the 33 isolates, 16 were classified as BVDV-1 and 17 as BVDV-2. Phylogenetic analysis of the PCR-amplified fragments from 14 isolates showed the presence of viruses belonging to the BVDV-1a, BVDV-1b, BVDV-1c, and BVDV-2 types. Antigenicity of some viruses belonging to both genotypes was studied by cross-seroneutralization, revealing great antigenic differences among them. It is concluded that BVDV viruses circulating in Chile are genetically and antigenically heterogeneous, comprising isolates of genogroups 1 and 2.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Molecular characterization of Mycoplasma synoviae isolated from broiler chickens of West Azarbaijan province by PCR of vlhA gene

Mycoplasma synoviae (MS) is a pathogen responsible for respiratory and locomotor disorders and causes major economic losses in poultry industry. Early and accurate diagnosis of MS infection plays a major role in control of the infection. This study was conducted to characterize Iranian field isolates of MS isolated from broiler chickens of West Azarbaijan province (Northwest of Iran), ...

متن کامل

Study of biological and molecular characterization of pepper-PVY isolated from Tehran pepper fields and it’s comparison with other PVY isolates

Abstract: Potato virus Y a type species of the genus potyvirus infects several crops in the family solanaceae. PVY isolated from field infected peppers was identified on the basis of host reaction, serological and molecular characterization. The result of ELISA, Immunoblot electrophoresis and Immuno Capture Reverse Transcription Polymerase Chain Reaction   (IC-RT-PCR) indicated that...

متن کامل

Culture and molecular characterization of phages isolated from rainbow trout farms and sewage treatment plants and investigation of their effects on Yersinia ruckeri

In the present study bacteriophages isolated from rainbow trout farms and sewage treatment plants were genetically identified and their effectiveness on Yersinia ruckeri isolates from clinical cases of red mouth disease was investigated.  Fish samples suspected to red mouth disease were collected from rainbow trout farms located in west Azerbaijan. Y. ruckeri, the causative agent of red mouth d...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Veterinary microbiology

دوره 115 1-3  شماره 

صفحات  -

تاریخ انتشار 2006